Perseus Proteomics have established a high sensitivity PTX3 detection method, which we expect to be a breakthrough biomarker capable of predicting patients' risk of atherosclerosis in the earliest stages.
We start selling our high sensitivity PTX3 ELISA kit “Human Pentraxin 3 / TSG-14 ELISA System” for research use only.
Assay Protocol
Sample preparation
●Plasma
Fresh or defrosted human plasma prepared with EDTA. Samples may be stored at ＜−20℃.
After collecting blood, centrifuge for 10 minutes at 3,000 rpm. Assay immediately or aliquot and store samples at ＜−20℃ within 2 hours.
Caution: Serum, heparin plasma and citrate plasma samples can NOT be used with this kit.
●Cell culture supernatants
•Remove particulates by centrifugation and assay immediately or aliquot and store samples at ＜−20℃.
Caution:Using culture supernatant containing serum (FBS) is not recommended, as there is some possibility of assay inhibition by FBS. If you use culture supernatant
containing FBS, please verify recovery prior to measuring samples. |
Performance cheracteristics --Lineality and Detection limit --
Lineality and Detection limit |
Representative calibration curve based on PTX3 calibrators of 0.5-20ng/mL.
The lower limit of detection is 0.1ng/mL. |
Performance cheracteristics --Spike and Recovery--
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Average recovery is 97.3. |
Performance cheracteristics --Cross reactivity--
Cross reactivity test |
Cross reactivity with CRP and SAP is ＜0.1ng/mL |
Performance cheracteristics --Reproducibility--
Intra-assay and Inter-assay |
Intra-assay within 4.1%, Inter-assay within 4.3%. |
Kit components
This kit contains the following assay components, sufficient for 96 wells.
•Anti-human PTX3 antibody precoated strip well plate, 1 (96 well)
•HRP conjugated anti-PTX3 antibody reagent, 1 vial (12 mL)
•Human PTX3 standards 0, 0.5, 1.0, 2.5, 5.0, 10, 20 ng/mL, 7 vials (0.1 mL),
•10X Wash buffer concentrate, 1 vial (100 mL)
•Dilution buffer, 1 vial (15 mL) containing preservative
•TMB solution, 1 vial (12 mL)
•Stop solution, 1 vial (12 mL)
•Adhesive plate covers, 3 sheets
•Instruction manual, 1
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PTX3 Antibodies
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Product Information
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