Diagnostic Agents

We develop extracorporeal diagnostic agents to quantify trace molecular biomarkers expressed by tumor cells or disease cells in the blood. These diagnostics will allow doctors to assess a patients’ likelihood of responding to a given therapy or monitor the progression of a patients’ disease state, thereby facilitating a more precise and effective choice from the available therapeutic options. We look at disease as a process from preservation through diagnostication, prognosis, and treatment rather than a state, and we will realize such “Theranostics”, as a new approach to disease management.

A vascular inflammation marker, Pentraxin 3 (PTX3/TSG-14)

2008/9/25 Pentraxin 3 appears to be an excellent predicative biomarker for acute coronary syndrome. Perseus Proteomics have established a high sensitivity PTX3 detection method(*1), which we expect to be a breakthrough biomarker capable of predicting patients' risk of atherosclerosis in the earliest stages. We start selling PTX3 ELISA System “Human Pentraxin3/TSG-14 ELISA System” for research use. About our “human Pentraxin3 / TSG-14 ELISA System”

An introduction of a vascular inflammatory marker, Pentraxin 3 (PTX3)

Many inflammatory mediators are closely-involved in atherosclerosis, plaque formation and rupture(*2). One of the most famous inflammation mediators is CRP(C-reactive protein) which has been widely used as an inflammatory biomarker for predicting the occurrence of future cardiovascular events. CRP and SAP (Serum Amyloid P component) are referred to as classical short pentraxins and are acute phase proteins produced from the liver in response to inflammatory mediators(*3-4) Pentraxin 3 (PTX3), a recently identified member of the pentraxin family, is referred to as a long pentraxin and contains a unique PTX3 domain not found in CRP or SAP. In contrast to CRP, PTX3 is produced from the major cell types involved in atherosclerotic lesions, namely vascular endothelial cells, vascular smooth muscle cells, macrophages, and nutrophils in response to inflammatory stimuli(*5-10). Moreover, CRP is produced by the liver and represents a systemic response to local inflammation, whereas PTX3 is rapidly produced directly from damaged tissues and directly reflects the inflammatory state of the vasculature. PTX3 levels have been reported to be significantly elevated in acute myocardial infarction(*11), and accordingly, statin treatment significantly decreases PTX3 levels(*12). Due to the fact that PTX3 is able to reflect ACS condition better than CRP, it is highly possible that PTX3 is a superior biomarker to predict future cardiovascular events. Contact PTX3 customer service by e-mail ptx3@ppmx.com

Amino acid sequence of PTX3 and other pentraxin family members Pentraxin in humoral immate immunity

Publications based on human Pentraxin 3/TSG-14 ELISA System

9　Int J Cardiol. [In Press, Available online] Prognostic value of pentraxin 3 in patients with chronic heart failure. Kotooka N, Inoue T, Aoki S, Anan M, Komoda H, Node K. 8　Dig Dis Sci. 2008 ; 53(7) : 1910-6. Increased Expression of Long Pentraxin PTX3 in Inflammatory Bowel Diseases. Kato S, Ochiai M, Sakurada T, Ohno S, Miyamoto K, Sagara M, Ito M, Takeuchi K, Imaki J, Itoh K, Yakabi K. 7　Atherosclerosis. 2008 ; 197(1) : 368-74. Pentraxin3 is a novel marker for stent-induced inflammation and neointimal thickening. Kotooka N, Inoue T, Fujimatsu D, Morooka T, Hashimoto S, Hikichi Y, Uchida T, Sugiyama A, Node K. 6　Am Heart J. 2008 ; 155(1) : 75-81. Pentraxin 3, a new marker for vascular inflammation, predicts adverse clinical outcomes in patients with heart failure. Suzuki S, Takeishi Y, Niizeki T, Koyama Y, Kitahara T, Sasaki T, Sagara M, Kubota I. 5　Atherosclerosis 2008 ; 197(1) : 368-74. Pentraxin3 is a novel marker for stent-induced inflammation and neointimal thickening Norihiko Kotooka, Teruo Inoue, Daisuke Fujimatsu, Toshifumi Morooka, Shigemsa Hashimoto, Yutaka Hikichi, Toshihiko Uchida, Akira Sugiyama, Koichi Node 4　J Pathol. 2008 ; 215(1) : 48-55. Expression of pentraxin 3 (PTX3) in human atherosclerotic lesions. Savchenko A, Imamura M, Ohashi R, Jiang S, Kawasaki T, Hasegawa G, Emura I, Iwanari H, Sagara M, Tanaka T, Hamakubo T, Kodama T, Naito M. 3　Cell Immunol. 2007 ; 248(2) : 86-94. Lipopolysaccharide induced expression of pentraxin 3 in human neutrophils and monocyte-derived macrophages. Imamura M, Kawasaki T, Savchenko AS, Ohashi R, Jiang S, Miyamoto K, Ito Y, Iwanari H, Sagara M, Tanaka T, Hamakubo T, Kodama T, Uchiyama M, Naito M. 2　Clin J Am Soc Nephrol. 2007 ; 2(5) : 889-97. Plasma pentraxin 3 in patients with chronic kidney disease: associations with renal function, protein-energy wasting, cardiovascular disease, and mortality. Tong M, Carrero JJ, Qureshi AR, Anderstam B, Heimburger O, Barany P, Axelsson J,　Alvestrand A, Stenvinkel P, Lindholm B, Suliman ME. 1　 Arterioscler Thromb Vasc Biol. 2007 ; 27(1) : 161-7. Establishment of a High Sensitivity Plasma Assay for Human Pentraxin3 as a Marker for Unstable Angina Pectoris Kenji Inoue, Akira Sugiyama, Patrick C. Reid, Yukio Ito, Katsumi Miyauchi, Sei Mukai, Mina Sagara, Kyoko Miyamoto, Hirokazu Satoh, Isao Kohno, Takeshi Kurata, Hiroshi Ota, Alberto Mantovani, Takao Hamakubo, Hiroyuki Daida, and Tatsuhiko Kodama

Reference

*1 Arteriosclerosis, Thrombosis, and Vascular Biology 2006 Establishment of a High Sensitivity Plasma Assay for Human Pentraxin3 as a Marker for Unstable Angina Pectoris Inoue K.et al . *2. Nature. 2000 Sep 14;407(6801):233-41. PMID: 11001066 Atherosclerosis. Lusis AJ. et al. *3. Immunol Today. 1994 Feb;15(2):74-80. PMID: 7512342 The acute phase response. Baumann H. et al. *4. Immunol Today. 1994 Feb;15(2):81-8. PMID: 8155266 The major acute phase reactants: C-reactive protein, serum amyloid P component and serum amyloid A protein Steel DM. et al. *5. J Immunol. 1994 Oct 15;153(8):3700-7. PMID: 7523502 Relationship of TSG-14 protein to the pentraxin family of major acute phase proteins. Lee GW. et al. *6. J Biol Chem. 1997 Dec 26;272(52):32817-23. PMID: 9407058 Multimer formation and ligand recognition by the long pentraxin PTX3. Bottazzi B. et al. *7. Blood. 1996 Mar 1;87(5):1862-72. PMID: 8634434 Cloning of mouse ptx3, a new member of the pentraxin gene family expressed at extrahepatic sites. Introna M. et al. *8. Annu Rev Immunol. 2005;23:337-66. Review. PMID: 15771574 Pentraxins at the crossroads between innate immunity, inflammation, matrix deposition, and female fertility. Garlanda C. et al. *9. J Biol Chem. 1997 Mar 28;272(13):8172-8. PMID: 9079634 Characterization of the promoter for the human long pentraxin PTX3. Role of NF-kappaB in tumor necrosis factor-alpha and interleukin-1beta regulation. Basile A, et al. *10. J Exp Med. 2007;204(4):793-804. The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps. Jaillon, S. et al. *11. Circulation. 2000 Aug 8;102(6):636-41. PMID: 10931803 PTX3, A prototypical long pentraxin, is an early indicator of acute myocardial infarction in humans. Peri G. et al. *12. J Atheroscler Thromb. 2002;9(4):178-83. PMID: 12226549 The effect of statins on mRNA levels of genes related to inflammation, coagulation, and vascular constriction in HUVEC. Human umbilical vein endothelial cells. Morikawa S, et al.

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About human Pentraxin3/TSG-14 ELISA System
Human Pentraxin3/TSG-14 ELISA System pamphlet
Human Pentraxin3/TSG-14 ELISA System instruction